解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (3 ): 297-303.doi: 0.3969/j.issn.0529-1356.2013.03.001

• 神经生物学 •    下一篇

酒精暴露对视网膜致畸作用和细胞凋亡的影响

李瑞玲 周洁 王强 刘彬 黄霞 马战友 邓锦波*   

  1. 河南大学护理学院神经生物学研究所，河南 开封 475004
  • 收稿日期:2012-03-12 修回日期:2012-05-23 出版日期:2013-06-06 发布日期:2013-07-16
  • 通讯作者: 邓锦波 E-mail:zhoujie4123@126.com
  • 基金资助:

    国家自然科学基金重点资助项目

Prenatal alcohol exposure inducing retinal teratogeny and cell apoptosis

  1. Institute of Neurobiology of Nursing College, He’nan University, He’nan  Kaifeng 475004, China
  • Received:2012-03-12 Revised:2012-05-23 Online:2013-06-06 Published:2013-07-16

摘要：

目的 探讨产前酒精暴露（PAE）对视网膜致畸作用和细胞凋亡的影响。方法 利用C57BL/6J小鼠建立孕期酒精暴露模型，用HE染色和
免疫荧光染色技术观察酒精对生后0d （P0）、P7、P14和P30 4个年龄点共72只子鼠的视网膜致畸性和细胞凋亡的影响。结果 高剂量酒精组中
视网膜的畸形率增加；在各剂量组Caspase-3、Caspase-8、Caspase-9 阳性细胞于内颗粒层中的表达与在节细胞层中的表达具有一致性，且具
有剂量依赖性(P<0.05)； TUNEL染色结果发现，与对照组相比，酒精组各年龄点的内颗粒层和节细胞层中细胞凋亡量均增加（P<0.05），提示
孕期酒精暴露诱导视网膜细胞发生凋亡时具有长时程效应。结论 视网膜畸形及细胞凋亡可能是导致PAE相关眼病的病理学基础。

关键词: 产前酒精暴露, 视网膜细胞, 发育, HE染色, 免疫荧光染色, 小鼠

Abstract:

Objective To investigate the effect of teratogeny and cell apoptosis on retina after prenatal alcohol exposure
(PAE).Methods A total of 72 mouse models of prenatal alcohol exposure were made. HE staining and immunofluorescent labeling
were carried out to visualize the retinal pathology and cell apoptosis. Results High prenatal alcohol exposure increased the
rate of retinal malformation; The expression of positive cells in inner granular layer(INL) was costistent with ganglial cell
layer(GCL) in the retina with dose-dependency (P<0.05); TUNEL showed in the retina INL and GCL, alcohol induced thecell apoptosis
with long-range effects (P<0.05). Conclusion Retinal malformation and cell apoptosis probably are the cause of eye diseases induced
by PAE.

Key words: Prenatal alcohol exposure, Retinal cell, Development, HE staining, Immunofluorescent labelin, Mouse